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During biodegradation, microorganisms that colonize the surface of the GAC, forming a biofilm, metabolize organic contaminants

The study protocol was approved by the Research Ethics Committee of the Faculty of Pharmacy, Damanhour University (Reference no: 1121PP46) and by the Ethics Committee on Human Research of the Faculty of Medicine, Alexandria University (Reference no: 0106942)

For samples frozen on holey carbon grids, the Vitrobot was maintained at 4LC with 100% humidity, using blotting times of 3.5Ls to 4.5Ls, blotting force of 0, and a waiting time of 10Ls